ISSN: 1300-0365
Dil: Türkçe
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Retinal Helix Pomatia Lektin Bağlanma Alanları: Altın İşaretlerne Ile Ultrastrüktürel İnceleme
Tongalp H.TEZEL*, Gülgün TEZEL*, İlhan GÜNALP**, Aysel ŞEFTALİOĞLU***
*Op.Dr. Ankara Numune Hastanesi 2. Göz Kliniği Başasistanı**Prf.Dr. Ankara ÜTF. Göz Hastalıkları ABD,***Prof.Dr. Hacettepe ÜTF. Histoloji-Embriyoloji ABD, ANKARA
HELIX POMATIA LECTIN BINDING SITES IN RABBIT RETINA: AN ULTRASTRUCTURAL STUDY WITH GOLD
LABELING
The glycolipids and glycoproteins in the membrane, intracellular and extracellular compartments of the retinal cells
participate in their normal physiologic processes and various pathologic responses. Lectins are proteins that has
remarkable specificity for certain haptane sugars that constitute a part of these molecules. In this study the distribution of
a-N-Acetyl D-galactosamine in the mammalian retina was investigated by using Helix pomatia lectin in the rabbit retina.
This investigation was carried by gold labeling method at the ultrastructural level. Gold particles were found scattered on
the photoreceptors outer segment plasma and disc membranes, as well as on the interphotoreceptor matrix. They also
appeared to be distributed in the axons of the nerve fiber layer, lysosomes of the retinal pigment epithelial cells and the
inner and outer collagenous layer of the Bruch's membrane. The identification of the sites of a-N-acetyl D-galactosamine
residues suggested that they may play a role In the recognition processes between phagocytic pigment epithelium cells
and the photoreceptors, as well as in the renewal of the disc membranes.Keywords: Cytochemistry, Helix pomatia lectin, Gold labeling electron microscopy
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