ISSN: 1300-0365
Dil: Türkçe
|
|
|
Keratocyte Loss After Different Methods Of De-epithelialization In Rabbits
Esin F. ERKİN*, Mine HEKİMGİL**, Yüksel ERKİN***, S. Sami İLKER****, Übeyt İNAN*****
* Yrd.Doç.Dr.,Celal Bayar Üniversitesi Tıp Fakültesi Göz Hastalıkları AD, MANİSA** Yrd.Doç.Dr.,Ege Üniversitesi Tıp Fakültesi Patoloji AD,*** Uz.Dr.,Dokuz Eylül Üniversitesi Tıp Fakültesi Anesteziyoloji AD, İZMİR**** Doç.Dr.,Celal Bayar Üniversitesi Tıp Fakültesi Göz Hastalıkları AD,***** Arş.Gör.Dr.,Celal Bayar Üniversitesi Tıp Fakültesi Göz Hastalıkları AD, MANİSA
Corneal de-epithelialization is performed for therapeutic purposes, for the management of intraoperative clouding and before photorefractive keratectomy. The viability of corneal stromal cells is related to the presence of epithelium. In this study the method that might cause the least early keratocyte loss and inflammatory reaction in the stroma after de-epithelialization of the rabbit cornea with various methods is searched. 20 eyes of 10 rabbits were included in the study. A 6 mm area in the central cornea was de-epithelialized using three different methods (direct mechanical, using cocaine or oxybuprocain embedded sponge), 2 eyes serving as controls. Following enucleation after 24 hours, corneas were evaluated histopathologically. When compared with control corneas, all de-epithelialized corneas showed a decrease in keratocytes and the presence of polymorphonuclear leukocytes (p<0.05). Keratocyte loss and leukocyte infiltration were seen the most with direct mechanical method, whereas they were seen less with de-epithelialization using cocain and oxybuprocain embedded sponge (p<0.05). There were no differences in leukocyte infiltration among groups. As a result, it is concluded that when de-epithelialization is required, use of cocain or oxybuprocain embedded sponge is more suitable than mechanical means using instruments like spatula or blade.Keywords: Cornea, de-epithelialization, Keratocyte,
RabbitTurkiye Klinikleri J Ophthalmol 1998, 7:13-17
|
|
|
|
|
