ISSN: 1300-0365
Dil: Türkçe
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A Direct Fluorescence Staining:acridine Orange In Diagnosis And Empirical Treatment Of Endophthalmitis
Aysu Karatay Arsan*, Nilgün Acar**, Esra Alp IşIk***, Seyhan B. Özkan****, Sunay Duman*****
* Op.Dr.,SB Ankara Hastanesi Göz Kliniği, Başasist.,** Uz.Dr.,SB Ankara Hastanesi Klinik Mikrobiyoloji Kliniği, Şef Yrd.,*** Uz.Dr.,SB Ankara Hastanesi Klinik Mikrobiyoloji Kliniği, Başasist.,**** Op.Dr.,SB Ankara Hastanesi Göz Kliniği, Uzm.,***** Op.Dr.,SB Ankara Hastanesi Göz Kliniği, Şefi, Ankara
Direct microscobic examination of intraocular specimens, particularly vitreus can provide a more rapid way to detect the responsible microorganism. The purpose of this study was to compare the sensitivity of acridine orange (AO) and Gram staining to determine whether AO offers any advantages. We made the direct microscobic examination and cultured the intraocular samples of 22 patients in whom acute postoperative endophthalmitis was diagnosed. Double smears of each specimen were prepared. After the AO-stained specimens had been examined on fluorescence microscopy, same specimens were stained with Gram. Overall, AO was more sensitive than the Gram stain (AO 82%, Gram stain 64%). While 19 of 22 cases were culture positive, no microorganism was identified in 3. We conclude that AO staining is a sensitive method especially for selected purulant specimens in which protein artefacts result in difficulties of interpretation. Reexamination of the positive smears with Gram stain to determine the morphology and Gram staining character of the responsible microorganism offers an additional advantage of AO staining.Keywords: Endophthalmitis, Gram stain, acridine orangeTurkiye Klinikleri J Ophthalmol 1999, 8:15-18
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