ISSN: 1300-0365
Dil: Türkçe
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ORIGINAL RESEARCH ARTICLES |
Effects Of Topical Vitamin-e And Ascorbic Acid On Keratocyte Apoptosis After Mechanical Epithelial Debridement In Rabbit Eyes
Dr. Şengül ÖZDEK,a Dr. Ali ALTINSOY,a Dr. Ufuk ADIGÜZEL,b Dr. Cem SEZER,cDr. Berati HASANREİSOĞLU,a Dr. Kamil BİLGİHANa
aGöz Hastalıkları AD, Gazi Üniversitesi Tıp Fakültesi, ANKARAbGöz Hastalıkları AD, Mersin Üniversitesi Tıp Fakültesi, MERSİNcPatoloji AD, Gazi Üniversitesi Tıp Fakültesi, ANKARA
Objective: To study the effect of vitamin-E and C on keratocyte apoptosis following mechanical epithelial debridement in rabbit corneas.
Material and Methods: Twenty New Zealand white rabbits were used divided into 4 groups and only one eye of each rabbit was deepithelialized mechanically within a 7mm zone by using a blunt (Kimura) spatula. Group 1 was not treated with any of the vitamins and acted as a control group. One drop of vitamin E (1IU/mg, 50IU/drop) was applied to the cornea 2-3 minutes before epithelial removal in group 2. Group 3 received topical vitamin-C (10% (w/v) ascorbic acid) just after epithelial removal and lastly group 4 was treated with topical vitamin-E before and vitamin-C after the epithelial debridement. Central corneal tissues were removed and TUNEL assay was used for the demonstration of the apoptotic keratocytes 4 hour after corneal surgery. PMN leukocyte densities were also assessed. ANOVA test was used for the statistical evaluation of apoptotic cells and PMN cells between all groups.
Results: Control group had the highest number of apoptotic keratocyte (ANOVA: p<0.05, Post Hoc test: p<0.05 for all three groups). On the other hand, there was no significant difference between each vitamin group (Post Hoc test: p>0.05). There was no statistically significant difference between groups in terms of PMN cell number (ANOVA: p>0.05).
Conclusion: Mechanical epithelial debridement induces keratocyte apoptosis which can be prevented by vitamins E and C at least partially. These agents may be the potential medications for modulation of corneal wound healing after refractive surgeries.Keywords: Keratocyte apoptosis, epithelial debridement, vitamin-E, ascorbic acidTurkiye Klinikleri J Ophthalmol 2004, 13:175-179
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